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KMID : 0613820110210111526
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Journal of Life Science
2011 Volume.21 No. 11 p.1526 ~ p.1531
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Localization of Translation Initiation Factors to the Postsynaptic Sites
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Choi Myoung-Kwon
Park Sung-Dong
Park In-Sick
Moon Il-Soo
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Abstract
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Local protein synthesis in neuronal dendrites is important for site-specific regulation of synaptic plasticity. In this study, we investigated whether translation initiation factors (eIFs) are present at the postsynaptic sites. High resolution confocal microscopy showed that the eIF4E and eIF4G (which bind the 5'-terminal mRNA cap), eIF5 (which is important during the 3' direction scanning to find an initiation codon), eIF6 (which mediates upregulation of translation by external stimuli), and eIF5A (which mediate translation upregulation under adverse conditions) were localized to the post-synaptic sites. Immunoblot and detergent extraction experiments also indicated that these eIFs were present in the synapse in association with the postsynaptic density (PSD). Our data provide evidence for the strategic positioning of eIFs at the postsynaptic site for initiation of translation in diverse situations.
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KEYWORD
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eIF, neuron, PSD, spine, translation
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